Study on Some Factors Affecting the Precision of Luminescence Analyses

Akiyoshi Taniguchi, Hidetaka Yuki, Yuzuru Hayashi

研究成果: Article

2 引用 (Scopus)

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The effects of the shape of the reaction vessel, the sample volume and the volume and speed of the reagent injection on the reproducibility of the chemiluminescence (CL) analyses were investigated with a laboratory-built automated injection device and a computer system. The suitable selections of the vessel diameter as well as the injection volume are shown to be necessary for the precise measurements of samples of given volume. Under the optimal mixing conditions, the choice of signal processings such as peak height measurements or fixed-time integration did not affect the reproducibility and any of the methods used could give accurate CL values. These principles for the precise measurement represent useful guides for luminometer designing and experiments for rapid, precise analyses.

元の言語English
ページ(範囲)3475-3480
ページ数6
ジャーナルChemical and Pharmaceutical Bulletin
34
発行部数8
DOI
出版物ステータスPublished - 1986
外部発表Yes

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ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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