Studying anti-oxidative properties of inclusion complexes of α-lipoic acid with γ-cyclodextrin in single living fission yeast by confocal Raman microspectroscopy

Hemanth Noothalapati*, Ryo Ikarashi, Keita Iwasaki, Tatsuro Nishida, Tomohiro Kaino, Keisuke Yoshikiyo, Keiji Terao, Daisuke Nakata, Naoko Ikuta, Masahiro Ando, Hiro o. Hamaguchi, Makoto Kawamukai, Tatsuyuki Yamamoto

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

α-lipoic acid (ALA) is an essential cofactor for many enzyme complexes in aerobic metabolism, especially in mitochondria of eukaryotic cells where respiration takes place. It also has excellent anti-oxidative properties. The acid has two stereo-isomers, R- and S- lipoic acid (R-LA and S-LA), but only the R-LA has biological significance and is exclusively produced in our body. A mutant strain of fission yeast, Δdps1, cannot synthesize coenzyme Q10, which is essential during yeast respiration, leading to oxidative stress. Therefore, it shows growth delay in the minimal medium. We studied anti-oxidant properties of ALA in its free form and their inclusion complexes with γ-cyclodextrin using this mutant yeast model. Both free forms R- and S-LA as well as 1:1 inclusion complexes with γ-cyclodextrin recovered growth of Δdps1 depending on the concentration and form. However, it has no effect on the growth of wild type fission yeast strain at all. Raman microspectroscopy was employed to understand the anti-oxidant property at the molecular level. A sensitive Raman band at 1602 cm−1 was monitored with and without addition of ALAs. It was found that 0.5 mM and 1.0 mM concentrations of ALAs had similar effect in both free and inclusion forms. At 2.5 mM ALAs, free forms inhibited the growth while inclusion complexes helped in recovered. 5.0 mM ALA showed inhibitory effect irrespective of form. Our results suggest that the Raman band at 1602 cm−1 is a good measure of oxidative stress in fission yeast.

本文言語English
ページ(範囲)237-243
ページ数7
ジャーナルSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
197
DOI
出版ステータスPublished - 2018 5 15

ASJC Scopus subject areas

  • 分析化学
  • 原子分子物理学および光学
  • 器械工学
  • 分光学

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