Substrate recognition of pre-tRNA by ribonuclease P---subsite model of natural ribozyme originated from Escherichia coli.

Akihiro Fujimoto*, Yo Kikuchi, Terumichi Tanaka

*この研究の対応する著者

研究成果: Article査読

抄録

Combination of cleavage site analysis and kinetic analysis of a series of shape variant of pre-tRNA substrate, we newly found two subsites which contribute to recognition of shape in substrate binding and catalysis by bacterial RNase P. Our data showed that the ribozyme traps the substrate by 5'- and 3'-end regions to form Michaelis complex, and after that the shape of the substrate is examined by other two subsites in the transition state. In the meeting, we will show the possibility that the S-domain can contribute to stabilize the transition state of the cleavage reaction of a pre-tRNA substrate.

本文言語English
ページ(範囲)35-36
ページ数2
ジャーナルNucleic acids symposium series (2004)
53
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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