Sucrose Induces Vesicle Accumulation and Autophagy

Takahiro Higuchi, Jun Nishikawa, Hiroko Inoue*

*この研究の対応する著者

    研究成果: Article査読

    20 被引用数 (Scopus)

    抄録

    It has been shown that the treatment of mammalian cells with sucrose leads to vacuole accumulation associated with lysosomes and upregulation of lysosomal enzyme expression and activity. Autophagy is an evolutionarily conserved homeostatic process by which cells deliver cytoplasmic material for degradation into lysosomes, thus it is probable that sucrose affects the autophagic activity. The role of sucrose in autophagy is unknown; however, another disaccharide, trehalose has been shown to induce autophagy. In the current study, we used mouse embryonic fibroblasts to investigate whether sucrose induces autophagy and whether vesicle formation is associated with autophagy. The results showed that sucrose induces autophagy while being accumulated within the endosomes/lysosomes. These vesicles were swollen and packed within the cytoplasm. Furthermore, trehalose and the trisaccharide raffinose, which are not hydrolyzed in mammalian cells, increased the rate of vesicles accumulation and LC3-II level (a protein marker of autophagy). However, fructose and maltose did not show the same effects. The correlation between the two processes, vesicle accumulation and autophagy induction, was confirmed by treatment of cells with sucrose plus invertase, or maltose plus acarbose - the α-glucosidase inhibitor - and by sucrose deprivation. Results also showed that vesicle accumulation was not affected by autophagy inhibition. Therefore, the data suggest that sucrose-induced autophagy through accumulation of sucrose-containing vesicles is caused by the absence of hydrolysis enzymes. J. Cell. Biochem. 116: 609-617, 2015.

    本文言語English
    ページ(範囲)609-617
    ページ数9
    ジャーナルJournal of Cellular Biochemistry
    116
    4
    DOI
    出版ステータスPublished - 2015 4 1

    ASJC Scopus subject areas

    • 生化学
    • 細胞生物学
    • 分子生物学

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