Oxyhemoglobin(oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide(H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. In this study, we constructed a suppression system of metHb formation within Hb-vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb solution by adding metHb/L-Tyr in the experiment of stepwise addition of H 2O 2. We also confirmed similar effect in Hb vesicles coencapsulating metHb/L-Tyr. Furthermore, it was also effective in vivo. We concluded that H 2O 2 was stably eliminated by the metHb/L-Tyr system in Hb-vesicles, and oxyHb was protected from H 2O 2 reaction.
|出版ステータス||Published - 2005 12 1|
|イベント||54th SPSJ Annual Meeting 2005 - Yokohama, Japan|
継続期間: 2005 5 25 → 2005 5 27
|Conference||54th SPSJ Annual Meeting 2005|
|Period||05/5/25 → 05/5/27|
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