Suppression of metHb formation in Hb-vesicles by glutathione

H. Yokohama, Y. Seino, J. E. Chung, H. Sakai, Shinji Takeoka, Hiroyuki Nishide, E. Tsuchida

研究成果: Article

抄録

To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.

元の言語English
ページ(範囲)868-871
ページ数4
ジャーナルJapanese Journal of Artificial Organs
23
発行部数3
出版物ステータスPublished - 1994

Fingerprint

Glutathione
Oxygen
Pyridoxal Phosphate
Tocopherols
Partial Pressure
Reducing Agents
Lipid Peroxidation
Reactive Oxygen Species
Phospholipids
Pressure

ASJC Scopus subject areas

  • Biophysics

これを引用

Yokohama, H., Seino, Y., Chung, J. E., Sakai, H., Takeoka, S., Nishide, H., & Tsuchida, E. (1994). Suppression of metHb formation in Hb-vesicles by glutathione. Japanese Journal of Artificial Organs, 23(3), 868-871.

Suppression of metHb formation in Hb-vesicles by glutathione. / Yokohama, H.; Seino, Y.; Chung, J. E.; Sakai, H.; Takeoka, Shinji; Nishide, Hiroyuki; Tsuchida, E.

:: Japanese Journal of Artificial Organs, 巻 23, 番号 3, 1994, p. 868-871.

研究成果: Article

Yokohama, H, Seino, Y, Chung, JE, Sakai, H, Takeoka, S, Nishide, H & Tsuchida, E 1994, 'Suppression of metHb formation in Hb-vesicles by glutathione', Japanese Journal of Artificial Organs, 巻. 23, 番号 3, pp. 868-871.
Yokohama, H. ; Seino, Y. ; Chung, J. E. ; Sakai, H. ; Takeoka, Shinji ; Nishide, Hiroyuki ; Tsuchida, E. / Suppression of metHb formation in Hb-vesicles by glutathione. :: Japanese Journal of Artificial Organs. 1994 ; 巻 23, 番号 3. pp. 868-871.
@article{89da13ea2ccd49cc9e6abf23f9b2bb84,
title = "Suppression of metHb formation in Hb-vesicles by glutathione",
abstract = "To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.",
keywords = "α-tocopherol, glutathione, Hb-vesicles, lipid peroxidation, metHb reduction",
author = "H. Yokohama and Y. Seino and Chung, {J. E.} and H. Sakai and Shinji Takeoka and Hiroyuki Nishide and E. Tsuchida",
year = "1994",
language = "English",
volume = "23",
pages = "868--871",
journal = "Japanese Journal of Artificial Organs",
issn = "0300-0818",
publisher = "Japanese Society for Artificial Organs and Tissues",
number = "3",

}

TY - JOUR

T1 - Suppression of metHb formation in Hb-vesicles by glutathione

AU - Yokohama, H.

AU - Seino, Y.

AU - Chung, J. E.

AU - Sakai, H.

AU - Takeoka, Shinji

AU - Nishide, Hiroyuki

AU - Tsuchida, E.

PY - 1994

Y1 - 1994

N2 - To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.

AB - To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.

KW - α-tocopherol

KW - glutathione

KW - Hb-vesicles

KW - lipid peroxidation

KW - metHb reduction

UR - http://www.scopus.com/inward/record.url?scp=0028228397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028228397&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0028228397

VL - 23

SP - 868

EP - 871

JO - Japanese Journal of Artificial Organs

JF - Japanese Journal of Artificial Organs

SN - 0300-0818

IS - 3

ER -