Suppressive effects of the leaf of Terminalia catappa L. on osteoclast differentiation in vitro and bone weight loss in vivo

Tomoyuki Koyama*, Chie Nakajima, Sogo Nishimoto, Masamichi Takami, Je Tae Woo, Kazunaga Yazawa

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Oral administration of Terminalia catappa extract (TCE; 1,000 mg/kg) for 5 wk suppressed bone weight loss and trabecular bone loss in ovariectomized mice. An in vitro experiment showed that TCE (1.3-20 μ g/mL) did not increase alkaline phosphatase activity, which would indicate osteoclast formation, in osteoblast-like 3T3-L1 cells. On the other hand, TCE (12.5 μ g/mL) markedly decreased the number of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells, which would indicate osteoclast formation, in a co-culture system (bone marrow cells/osteoblastic UAMS-32 cells). A detailed analysis of the stages of osteoclast differentiation revealed that TCE mainly suppressed the differentiation of bone marrow mononuclear cells into osteoclast progenitor cells in the presence of MCSF and TGF- β. An additional experiment using fractionated TCE revealed that the watersoluble fraction suppressed the bone weight loss in OVX-mice and osteoclast differentiation in vitro. Therefore, the suppressive effects of TCE on bone weight loss in mice might be due to the suppressive effects of highly polar components on the early stage of osteoclast differentiation.

本文言語English
ページ(範囲)129-135
ページ数7
ジャーナルJournal of Nutritional Science and Vitaminology
58
2
DOI
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • 医学(その他)
  • 栄養および糖尿病

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