Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

Kotaro Oka, Hiroko Ikeshima, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

研究成果: Article査読

14 被引用数 (Scopus)

抄録

We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m2.

本文言語English
ページ(範囲)771-775
ページ数5
ジャーナルPlant and Cell Physiology
29
5
出版ステータスPublished - 1988 7
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生態学
  • 細胞生物学
  • 生理学
  • 植物科学

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