A novel hydrophilic method of the polyurea film surface at room temperature was developed using vacuum ultraviolet (VUV) light 172 nm in wavelength. The treated film has highly hydrophilic surface for long time (∼ 2 months). Hydrophilic surface is stable and is kept even after very hard condition of ultrasonic cleaning in de-ionized water. The polyurea film was applied for PMMA (polymethylmethacrylate) blood analysis chip. The performance of the surface treatment was evaluated by actual human blood measurements. The new chip can reduce the adhesion of platelets and white blood cells, even after ultrasonic cleaning in surfactant induced water. This result indicates that this chip can be reused after washing.