Square-millimeter-sized free-floating translucent films are formed in physiological buffer by multiway connections between biotinylated collagen-like triple-helical peptides and avidin. Although the compositions of the films are almost constant, regardless of the ratios of the components loaded, their thicknesses can be controlled by the concentrations of the components. The film surfaces can be further modified by taking advantage of exposed biotin (or avidin) functionalities. The self-assembled films could serve as novel materials in biomedical and biosensing applications.
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