Surface plasmon resonance sensors utilizing microfabricated channels

Motohiro Furuki*, Jun Kameoka, Harold G. Craighead, Michael S. Isaacson


研究成果: Article査読

27 被引用数 (Scopus)


Surface plasmon resonance (SPR) scanning and imaging measurements have many applications in chemical and biological sensing. In this paper, we have used SPR to study protein adsorption onto chemically modified gold surfaces. We present a method for using a patterned photobiotin probe in microfluidic channels for sensing proteins with SPR. Each flow cell in a chip is 15 μm × 90 μm × 6 mm, giving a sample volume of 8 nl. The flow cell is created using conventional photolithographic and etching techniques, employing an adhesive UV resin to hold a fused silica coverslip in place. To improve the selectivity and label proteins, photobiotin patterns are modified by UV illumination at 365 nm onto self-assembled monolayers (SAMs) of cysteamine on gold in the fluidic channels. The specific adsorption of the protein avidin onto the photobiotin layer is monitored with the SPR scanning technique, which is extremely sensitive to the index of refraction of the adjacent medium.

ジャーナルSensors and Actuators, B: Chemical
出版ステータスPublished - 2001 9月 25

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学


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