Sustainable antimicrobial effect of silver sulfadiazine-loaded nanosheets on infection in a mouse model of partial-thickness burn injury

Keisuke Ito, Akihiro Saito, Toshinori Fujie, Keisuke Nishiwaki, Hiromi Miyazaki, Manabu Kinoshita, Daizoh Saitoh, Shinya Ohtsubo, Shinji Takeoka

研究成果: Article査読

49 被引用数 (Scopus)

抄録

Partial-thickness burn injury has the potential for reepithelialization and heals within 3 weeks. If the wound is infected by bacteria before reepithelization, however, the depth of disruption increases and the lesion easily progresses to the full-thickness dermal layers. In the treatment of partial-thickness burn injury, it is important to prevent the wound area from bacterial infection with an antimicrobial dressing. Here, we have tested the antimicrobial properties of polymeric ultra-thin films composed of poly(lactic acid) (termed "PLA nanosheets"), which have high flexibility, adhesive strength and transparency, and silver sulfadiazine (AgSD), which exhibits antimicrobial efficacy. The AgSD-loaded nanosheet released Ag+ for more than 3 days, and exerted antimicrobial efficacy against methicillin-resistant Staphylococcus aureus (MRSA) in an in vitro Kirby-Bauer test. By contrast, a cell viability assay indicated that the dose of AgSD used in the PLA nanosheets did not show significant cytotoxicity toward fibroblasts. In vivo evaluation using a mouse model of infection in a partial-thickness burn wound demonstrated that the nanosheet significantly reduced the number of MRSA bacteria on the lesion (more than 105-fold) and suppressed the inflammatory reaction, thereby preventing a protracted wound healing process.

本文言語English
論文番号3734
ページ(範囲)87-95
ページ数9
ジャーナルActa Biomaterialia
24
DOI
出版ステータスPublished - 2015 9 15

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

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