Switching of cell growth/detachment on heparin-functionalized thermoresponsive surface for rapid cell sheet fabrication and manipulation

Yoshinori Arisaka, Jun Kobayashi, Masayuki Yamato, Yoshikatsu Akiyama, Teruo Okano*

*この研究の対応する著者

    研究成果: Article査読

    55 被引用数 (Scopus)

    抄録

    Heparin-functionalized poly(N-isopropylacrylamide- co-2-carboxyisopropylacrylamide) [P(IPAAm- co-CIPAAm)] grafted surface was designed for the switching of cell growth/detachment, achieved by the regulation of affinity binding between basic fibroblast growth factor (bFGF) and immobilized heparin through the temperature-dependent conformational change of grafted P(IPAAm- co-CIPAAm) chains. At 37 °C, bFGF-bound heparin-thermoresponsive surfaces were able to hold the two- to three-fold number of mouse fibroblast (NIH/3T3) cells than both bFGF-physisorbed surface and PIPAAm surface with soluble bFGF after a 3-day cultivation. Bound bFGF via heparin on shrunken grafted P(IPAAm- co-CIPAAm) chains at 37 °C was able to reinforce the formation and stabilization of bFGF-FGF receptor complex, although the activity of physisorbed bFGF on PIPAAm-grafted surfaces was decreased by non-specific and randomly oriented adsorption. At 20 °C, the cultured NIH/3T3 cell sheet with bFGF detached from heparin-functionalized thermoresponsive surface. The release of bFGF from the surfaces was induced by reducing the affinity binding between bFGF and immobilized-heparin due to increasing the mobility of the swollen grafted P(IPAAm- co-CIPAAm) chains. Therefore, heparin-functionalized thermoresponsive surface was able to enhance cell proliferation, and confluent cells detached themselves as a contiguous cell sheet due to switching cell growth by changing temperature. A cell culture system using this surface is useful for rapid cell sheet fabrication and manipulation.

    本文言語English
    ページ(範囲)4214-4222
    ページ数9
    ジャーナルBiomaterials
    34
    17
    DOI
    出版ステータスPublished - 2013 6月

    ASJC Scopus subject areas

    • 生体材料
    • バイオエンジニアリング
    • セラミックおよび複合材料
    • 材料力学
    • 生物理学

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