Synaptic function and neuropathological disease revealed by quantum dot-single-particle tracking

Hiroko Bannai*, Takafumi Inoue, Matsumi Hirose, Fumihiro Niwa, Katsuhiko Mikoshiba

*この研究の対応する著者

研究成果: Chapter

3 被引用数 (Scopus)

抄録

Quantum dot-single-particle tracking (QD-SPT) is a super-resolution imaging technique that uses semiconductor nanocrystal quantum dots as fluorescent probes and is a powerful tool for analyzing protein and lipid behavior in the plasma membrane. Recent QD-SPT experiments have provided critical insight into the mechanism and physiological relevance of membrane self-organization in neurons and astrocytes in the brain. The mobility of some membrane molecules may become abnormal in cellular models of epilepsy and Alzheimer’s disease. Based on these findings, we propose that the behavior of membrane molecules reflects the condition of neurons in pathological disease states. In this chapter, we describe the latest, simple QD-SPT technique, which is feasible with epifluorescence microscopy and dissociated cell cultures.

本文言語English
ホスト出版物のタイトルNeuromethods
出版社Humana Press Inc.
ページ131-155
ページ数25
DOI
出版ステータスPublished - 2020

出版物シリーズ

名前Neuromethods
154
ISSN(印刷版)0893-2336
ISSN(電子版)1940-6045

ASJC Scopus subject areas

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)
  • 精神医学および精神衛生

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