@inbook{06bb8ccb293d475dac35948430e7d633,
title = "Synaptic function and neuropathological disease revealed by quantum dot-single-particle tracking",
abstract = "Quantum dot-single-particle tracking (QD-SPT) is a super-resolution imaging technique that uses semiconductor nanocrystal quantum dots as fluorescent probes and is a powerful tool for analyzing protein and lipid behavior in the plasma membrane. Recent QD-SPT experiments have provided critical insight into the mechanism and physiological relevance of membrane self-organization in neurons and astrocytes in the brain. The mobility of some membrane molecules may become abnormal in cellular models of epilepsy and Alzheimer{\textquoteright}s disease. Based on these findings, we propose that the behavior of membrane molecules reflects the condition of neurons in pathological disease states. In this chapter, we describe the latest, simple QD-SPT technique, which is feasible with epifluorescence microscopy and dissociated cell cultures.",
keywords = "Astrocyte, Dissociated culture, Ion channel, Lateral diffusion, Lipids, Membrane molecules, Neuron, Quantum dot, Receptor, Single-particle tracking, Synapse",
author = "Hiroko Bannai and Takafumi Inoue and Matsumi Hirose and Fumihiro Niwa and Katsuhiko Mikoshiba",
note = "Funding Information: Grant support: JST/PRESTO (grant number JPMJPR15F8, Japan); JSPS/KAKENHI (grant numbers JP18H05414, JP17H05710, JP16K07316), Takeda Foundation. The authors would like to deeply thank Maxime Dahan, Sabine L?vi, Misa Arizono, and Antoine Triller for establishing this protocol. We thank Misa Kanatani and Akihiko Takashima for providing QD655-GluA1 data for this chapter. Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-0716-0532-5_7",
language = "English",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "131--155",
booktitle = "Neuromethods",
}