Synchronised expressions of LPXRFamide peptide and its receptor genes: Seasonal, diurnal and circadian changes during spawning period in grass puffer

M. Shahjahan, T. Ikegami, T. Osugi, K. Ukena, H. Doi, A. Hattori, Kazuyoshi Tsutsui, H. Ando*

*この研究の対応する著者

    研究成果: Article査読

    83 被引用数 (Scopus)

    抄録

    Among the RFamide peptide family, the LPXRFamide peptide (LPXRFa) group regulates the release of various pituitary hormones and, recently, LPXRFa genes were found to be regulated by photoperiod via melatonin. As a first step towards investigating the role of LPXRFa on reproductive function in grass puffer (Takifugu niphobles), which spawns in semilunar cycles, genes encoding LPXRFa and its receptor (LPXRFa-R) were cloned, and seasonal, diurnal and circadian changes in their absolute amounts of mRNAs in the brain and pituitary were examined by quantitative real-time polymerase chain reaction. The grass puffer LPXRFa precursor contains two putative RFamide peptides and one possible RYamide peptide. LPXRFa and LPXRFa-R genes were extensively expressed in the diencephalon and pituitary. The expression levels of both genes were significantly elevated during the spawning periods in both sexes in the brain and pituitary, although they were low in the spawning fish just after releasing eggs and sperm. The treatment of primary pituitary cultures with goldfish LPXRFa increased the amounts of follicle-stimulating hormone β- and luteinising hormone β-subunit mRNAs. In the diencephalon, LPXRFa and LPXRFa-R genes showed synchronised diurnal and circadian variations with one peak at zeitgeber time 3 and circadian time 15, respectively. The correlated expression patterns of LPXRFa and LPXRFa-R genes in the diencephalon and pituitary and the possible stimulatory effects of LPXRFa on gonadotrophin subunit gene expression suggest the functional significance of the LPXRFa and LPXRFa-R system in the regulation of lunar-synchronised spawning of grass puffer.

    本文言語English
    ページ(範囲)39-51
    ページ数13
    ジャーナルJournal of Neuroendocrinology
    23
    1
    DOI
    出版ステータスPublished - 2011 1月

    ASJC Scopus subject areas

    • 内分泌学
    • 内分泌学、糖尿病および代謝内科学
    • 内分泌系および自律システム
    • 細胞および分子神経科学

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