Synthesis and application of fluorescein- and biotin-labeled molecular probes for the chemokine receptor CXCR4

Shinya Oishi; Ryo Masuda; Barry Evans; Satoshi Ueda; Yukiko Goto; Hiroaki Ohno; Akira Hirasawa; Gozoh Tsujimoto; Zixuan Wang; Stephen C. Peiper; Takeshi Naito; Eiichi Kodama; Masao Matsuoka; Nobutaka Fujii

doi:10.1002/cbic.200700761

Synthesis and application of fluorescein- and biotin-labeled molecular probes for the chemokine receptor CXCR4

Shinya Oishi^*, Ryo Masuda, Barry Evans, Satoshi Ueda, Yukiko Goto, Hiroaki Ohno, Akira Hirasawa, Gozoh Tsujimoto, Zixuan Wang, Stephen C. Peiper, Takeshi Naito, Eiichi Kodama, Masao Matsuoka, Nobutaka Fujii

^*この研究の対応する著者

研究成果: Article › 査読

36 被引用数 (Scopus)

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The design, synthesis, and bioevaluation of fluorescence- and biotin-labeled CXCR4 antagonists are described. The modification of D-Lys8 at an ε-amino group in the peptide antagonist Ac-TZ14011 derived from polyphemusin II had no significant influence on the potent binding of the peptide to the CXCR4 receptor. The application of the labeled peptides in flow cytometry and confocal microscopy studies demonstrated the selectivity of their binding to the CXCR4 receptor, but not to CXCR7, which was recently reported to be another receptor for stromal cell-derived factor 1 (SDF-1)/CXCL12.

本文言語	English
ページ（範囲）	1154-1158
ページ数	5
ジャーナル	ChemBioChem
巻	9
号	7
DOI	https://doi.org/10.1002/cbic.200700761
出版ステータス	Published - 2008 5月 5
外部発表	はい

ASJC Scopus subject areas

生化学
分子医療
分子生物学
有機化学

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10.1002/cbic.200700761

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title = "Synthesis and application of fluorescein- and biotin-labeled molecular probes for the chemokine receptor CXCR4",

abstract = "The design, synthesis, and bioevaluation of fluorescence- and biotin-labeled CXCR4 antagonists are described. The modification of D-Lys8 at an ε-amino group in the peptide antagonist Ac-TZ14011 derived from polyphemusin II had no significant influence on the potent binding of the peptide to the CXCR4 receptor. The application of the labeled peptides in flow cytometry and confocal microscopy studies demonstrated the selectivity of their binding to the CXCR4 receptor, but not to CXCR7, which was recently reported to be another receptor for stromal cell-derived factor 1 (SDF-1)/CXCL12.",

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author = "Shinya Oishi and Ryo Masuda and Barry Evans and Satoshi Ueda and Yukiko Goto and Hiroaki Ohno and Akira Hirasawa and Gozoh Tsujimoto and Zixuan Wang and Peiper, {Stephen C.} and Takeshi Naito and Eiichi Kodama and Masao Matsuoka and Nobutaka Fujii",

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month = may,

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doi = "10.1002/cbic.200700761",

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AU - Oishi, Shinya

AU - Masuda, Ryo

AU - Evans, Barry

AU - Ueda, Satoshi

AU - Goto, Yukiko

AU - Ohno, Hiroaki

AU - Hirasawa, Akira

AU - Tsujimoto, Gozoh

AU - Wang, Zixuan

AU - Peiper, Stephen C.

AU - Naito, Takeshi

AU - Kodama, Eiichi

AU - Matsuoka, Masao

AU - Fujii, Nobutaka

PY - 2008/5/5

Y1 - 2008/5/5

N2 - The design, synthesis, and bioevaluation of fluorescence- and biotin-labeled CXCR4 antagonists are described. The modification of D-Lys8 at an ε-amino group in the peptide antagonist Ac-TZ14011 derived from polyphemusin II had no significant influence on the potent binding of the peptide to the CXCR4 receptor. The application of the labeled peptides in flow cytometry and confocal microscopy studies demonstrated the selectivity of their binding to the CXCR4 receptor, but not to CXCR7, which was recently reported to be another receptor for stromal cell-derived factor 1 (SDF-1)/CXCL12.

AB - The design, synthesis, and bioevaluation of fluorescence- and biotin-labeled CXCR4 antagonists are described. The modification of D-Lys8 at an ε-amino group in the peptide antagonist Ac-TZ14011 derived from polyphemusin II had no significant influence on the potent binding of the peptide to the CXCR4 receptor. The application of the labeled peptides in flow cytometry and confocal microscopy studies demonstrated the selectivity of their binding to the CXCR4 receptor, but not to CXCR7, which was recently reported to be another receptor for stromal cell-derived factor 1 (SDF-1)/CXCL12.

KW - CXCR4 antagonists

KW - Cell imaging

KW - Chemokine receptors

KW - Fluorescent probes

KW - Peptides

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DO - 10.1002/cbic.200700761

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JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

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Synthesis and application of fluorescein- and biotin-labeled molecular probes for the chemokine receptor CXCR4

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