Synthesis and characterization of poly(ethylene glycol)-glutathione conjugate self-assembled nanoparticles for antioxidant delivery

Sharlene R. Williams, Benjamin S. Lepene, Craig D. Thatcher, Timothy Edward Long

研究成果: Article

53 引用 (Scopus)

抄録

Functional oligomers based on glutathione (GSH) and poly(ethylene glycol) diacrylate (PEGDA) were synthesized via Michael addition. Well-defined, spherical nanoparticle self-assembly was confirmed via dynamic light scattering and transmission electron microscopy. In addition, a series of Michael addition oligomers containing GSH were prepared with various molecular weights of poly(ethylene glycol) (PEG). Thermal analysis indicated that the oligomers were thermally stable to ∼160 °C, and the T g increased as the PEG molecular weight increased. In addition, thiol-terminated PEG was synthesized and reacted with GSH to form disulfide-linked oligomers to probe potential antioxidant therapies. SH-SY5Y cells were utilized in cell culture experiments, and hydrogen peroxide induced oxidative stress on the cells. Disulfide-linked GSH oligomers were 100% effective at protecting SH-SY5Y cells from oxidative stress, whereas the Michael addition GSH oligomers did not offer protection.

元の言語English
ページ(範囲)155-161
ページ数7
ジャーナルBiomacromolecules
10
発行部数1
DOI
出版物ステータスPublished - 2009 1 12
外部発表Yes

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Antioxidants
Oligomers
Polyethylene glycols
Nanoparticles
Oxidative stress
Disulfides
Molecular weight
Dynamic light scattering
Light transmission
Cell culture
Sulfhydryl Compounds
Hydrogen peroxide
Self assembly
Thermoanalysis
Hydrogen Peroxide
Glutathione
poly(ethylene glycol)-glutathione conjugate
Transmission electron microscopy
Experiments

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

これを引用

Synthesis and characterization of poly(ethylene glycol)-glutathione conjugate self-assembled nanoparticles for antioxidant delivery. / Williams, Sharlene R.; Lepene, Benjamin S.; Thatcher, Craig D.; Long, Timothy Edward.

:: Biomacromolecules, 巻 10, 番号 1, 12.01.2009, p. 155-161.

研究成果: Article

Williams, Sharlene R. ; Lepene, Benjamin S. ; Thatcher, Craig D. ; Long, Timothy Edward. / Synthesis and characterization of poly(ethylene glycol)-glutathione conjugate self-assembled nanoparticles for antioxidant delivery. :: Biomacromolecules. 2009 ; 巻 10, 番号 1. pp. 155-161.
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