Synthesis of cationic manganese porphyrin bearing alkylsulfonio groups and evaluation of their antioxidant activities

Makoto Yuasa, Kenichi Oyaizu, Hidenori Murata, Masayasu Komuro, Ryota Awa, Ayumi Ohkubo

研究成果: Article

3 引用 (Scopus)

抄録

A water-soluble cationic 5, 10, 15, 20-tetrakis(2-dimethylsulfoniophenyl)-porphinatomanganese(III) ion (MnT2M(2)SuP) and a 5, 10, 15, 20-tetrakis(4-dimethylsulfoniophenyl)porphinatomanganese(III) ion (MnT4M(2)SuP) were synthesized as superoxide dismutase (SOD) mimics which were introduced into PEG-liposome composed of dimyristoylphosphatidylcholine (DMPC) and Pluronic F-68 to examine the effect of the liposome on the capacity for use as drug delivery system (DDS) to maintain and perpetuate blood circulation. Fluorescence spectra in pseudo blood circulation experiments indicated that MnT4M(2)SuP continued to be bundled in PEG-liposome, while fluorescence from cross-section of cell observed by confocal laser scanning microscope indicated that PEG-liposome was ingested into a cell. SOD activity was determined by stopped-flow analysis, which allowed the determination of k(cat) values for the reaction of the metalloporphyrins with superoxide anion radical (.O(2)(-)). Solution of PEG-liposome loaded with MnT2M(2)SuP or MnT4M(2)SuP were the most effective catalyst as a SOD mimic to decompose .O(2)(-) at second-order rate constants of 3.5-4.5 x 10(7) M(-1)s(-1).

元の言語English
ページ(範囲)95-101
ページ数7
ジャーナルJournal of Oleo Science
56
発行部数2
出版物ステータスPublished - 2007
外部発表Yes

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Bearings (structural)
Liposomes
Porphyrins
Manganese
Antioxidants
Polyethylene glycols
Superoxide Dismutase
Blood Circulation
Hemodynamics
Superoxides
Fluorescence
Metalloporphyrins
Ions
Dimyristoylphosphatidylcholine
Poloxamer
Drug Delivery Systems
Rate constants
Lasers
Microscopes
Negative ions

ASJC Scopus subject areas

  • Medicine(all)

これを引用

Synthesis of cationic manganese porphyrin bearing alkylsulfonio groups and evaluation of their antioxidant activities. / Yuasa, Makoto; Oyaizu, Kenichi; Murata, Hidenori; Komuro, Masayasu; Awa, Ryota; Ohkubo, Ayumi.

:: Journal of Oleo Science, 巻 56, 番号 2, 2007, p. 95-101.

研究成果: Article

Yuasa, Makoto ; Oyaizu, Kenichi ; Murata, Hidenori ; Komuro, Masayasu ; Awa, Ryota ; Ohkubo, Ayumi. / Synthesis of cationic manganese porphyrin bearing alkylsulfonio groups and evaluation of their antioxidant activities. :: Journal of Oleo Science. 2007 ; 巻 56, 番号 2. pp. 95-101.
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AU - Awa, Ryota

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