Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

Ngang Heok Tang, Naoyuki Okada, Chii Shyang Fong, Kunio Arai, Masamitsu Sato, Takashi Toda

    研究成果: Article

    11 引用 (Scopus)

    抄録

    The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

    元の言語English
    ページ(範囲)2814-2821
    ページ数8
    ジャーナルFEBS Letters
    588
    発行部数17
    DOI
    出版物ステータスPublished - 2014 8 25

    Fingerprint

    Spindle Pole Bodies
    Centrosome
    Spindle Apparatus
    Schizosaccharomyces
    Yeast
    Poles
    Fungi
    Proteins
    Amino Acids

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Genetics
    • Molecular Biology
    • Structural Biology

    これを引用

    Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. / Tang, Ngang Heok; Okada, Naoyuki; Fong, Chii Shyang; Arai, Kunio; Sato, Masamitsu; Toda, Takashi.

    :: FEBS Letters, 巻 588, 番号 17, 25.08.2014, p. 2814-2821.

    研究成果: Article

    Tang, Ngang Heok ; Okada, Naoyuki ; Fong, Chii Shyang ; Arai, Kunio ; Sato, Masamitsu ; Toda, Takashi. / Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. :: FEBS Letters. 2014 ; 巻 588, 番号 17. pp. 2814-2821.
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    abstract = "The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.",
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    AU - Tang, Ngang Heok

    AU - Okada, Naoyuki

    AU - Fong, Chii Shyang

    AU - Arai, Kunio

    AU - Sato, Masamitsu

    AU - Toda, Takashi

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    AB - The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

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    KW - Fission yeast

    KW - Pericentrin family

    KW - Spindle microtubule

    KW - Spindle pole body

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