Temperature dependence of the flexural rigidity of single microtubules

Kenji Kawaguchi*, Shin'ichi Ishiwata, Toshihide Yamashita

*この研究の対応する著者

    研究成果: Article査読

    24 被引用数 (Scopus)

    抄録

    Although the flexural rigidity of a microtubule has previously been estimated by various methods, its temperature dependence has never been systematically examined. Here, we measured the flexural rigidity of a single taxol-stabilized microtubule from thermal fluctuation of the free end of a microtubule, the other end of which was fixed, at different temperatures; the results showed that the flexural rigidity is 2.54 × 10-24 N m2 independent of temperature in the range of 20-35 °C. Next, we applied temperature pulse microscopy (TPM) [K. Kawaguchi, S. Ishiwata, Thermal activation of single kinesin molecules with temperature pulse microscopy. Cell Motil. Cytoskeleton 49 (2001) 41-47; H. Kato, T. Nishizaka, T. Iga, K. Kinosita Jr., S. Ishiwata, Imaging of thermal activation of actomyosin motors. Proc. Natl. Acad. Sci. USA 96 (1999) 9602-9606], which created the temperature gradient (1-2 °C/μm) along a microtubule gliding on kinesins in the presence of ATP. As a result, the gliding microtubule was buckled between two interacting kinesin molecules, when the microtubule had been propelled faster by the rear kinesin (higher temperature) and slower by the front one (lower temperature). By estimating the critical force to induce buckling of a microtubule, the flexural rigidity of a microtubule was estimated to be (2.7-7.8) × 10-24 N m2, which was in good agreement with the value determined above. We discuss the buckling process based on the temperature dependence of the force-velocity relationship of kinesin motility.

    本文言語English
    ページ(範囲)637-642
    ページ数6
    ジャーナルBiochemical and Biophysical Research Communications
    366
    3
    DOI
    出版ステータスPublished - 2008 2 15

    ASJC Scopus subject areas

    • 生化学
    • 生物理学
    • 分子生物学

    フィンガープリント

    「Temperature dependence of the flexural rigidity of single microtubules」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル