Temporal perturbation of tyrosine phosphoproteome dynamics reveals the system-wide regulatory networks

Masaaki Oyama*, Hiroko Kozuka-Hata, Shinya Tasaki, Kentaro Semba, Seisuke Hattori, Sumio Sugano, Jun Ichiro Inoue, Tadashi Yamamoto

*この研究の対応する著者

研究成果査読

49 被引用数 (Scopus)

抄録

Signal transduction systems are known to widely regulate complex biological events such as cell proliferation and differentiation. Because phosphotyrosine-dependent networks play a key role in transmitting signals, a comprehensive and fine description of their dynamic behavior can lead us to systematically analyze the regulatory mechanisms that result in each biological effect. Here we established a mass spectrometry-based framework for analyzing tyrosine phosphoproteome dynamics through temporal network perturbation. A highly time-resolved description of the epidermal growth factor-dependent signaling pathways in human A431 cells revealed a global view of their multiphase network activation, comprising a spike signal transmission within 1 min of ligand stimulation followed by the prolonged activation of multiple Src-related molecules. Temporal perturbation of Src family kinases with the corresponding inhibitor PP2 in the prolonged activation phase led to the down-regulation of the molecules related to cell adhesion and receptor degradation, whereas the canonical cascades as well as the epidermal growth factor receptor relatively maintained their activities. Our methodology provides a system-wide view of the regulatory network clusters involved in signal transduction that is essential to refine the literature-based network structures for a systems biology analysis.

本文言語English
ページ(範囲)226-231
ページ数6
ジャーナルMolecular and Cellular Proteomics
8
2
DOI
出版ステータスPublished - 2009 2

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 分子生物学

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