Tepidicaulis Marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions

Mio Takeuchi, Takao Yamagishi, Yoichi Kamagata, Kenshiro Oshima, Masahira Hattori, Taiki Katayama, Satoshi Hanada, Hideyuki Tamaki, Katsumi Marumo, Hiroto Maeda, Munetomo Nedachi, Wataru Iwasaki, Yuichi Suwa, Susumu Sakata

研究成果: Article

2 引用 (Scopus)

抄録

A moderately thermophilic, aerobic, stalked bacterium (strain MA2<sup>T</sup>) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2<sup>T</sup>was most closely related to the genera Rhodobium, Parvibaculum, and Rhodoligotrophos (92–93% similarity) within the class Alphaproteobacteria. Strain MA2T was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16– 48 ºC (optimum growth at 42 ºC). This strain required yeast extract and NaCl (>1%, w/v) for growth, tolerated up to 11% (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C<inf>18: 1</inf>ω7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2<sup>T</sup>performed denitrification and produced N<inf>2</inf>O from nitrate under strictly microaerobic conditions. Strain MA2<sup>T</sup>possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2<sup>T</sup>(=NBRC 109643<sup>T</sup>=DSM 27167<sup>T</sup>) as the type strain of the species.

元の言語English
ページ(範囲)1749-1754
ページ数6
ジャーナルInternational Journal of Systematic and Evolutionary Microbiology
65
発行部数6
DOI
出版物ステータスPublished - 2015 6 1
外部発表Yes

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Nitrous Oxide
nitrous oxide
Nitrates
Nitrate Reductase
nitrates
nitrate
Bacteria
bacterium
gene
new species
bacteria
Growth
Geologic Sediments
Alphaproteobacteria
Denitrification
Aerobic Bacteria
Base Composition
rRNA Genes
Genes
marine sediment

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

これを引用

Tepidicaulis Marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions. / Takeuchi, Mio; Yamagishi, Takao; Kamagata, Yoichi; Oshima, Kenshiro; Hattori, Masahira; Katayama, Taiki; Hanada, Satoshi; Tamaki, Hideyuki; Marumo, Katsumi; Maeda, Hiroto; Nedachi, Munetomo; Iwasaki, Wataru; Suwa, Yuichi; Sakata, Susumu.

:: International Journal of Systematic and Evolutionary Microbiology, 巻 65, 番号 6, 01.06.2015, p. 1749-1754.

研究成果: Article

Takeuchi, M, Yamagishi, T, Kamagata, Y, Oshima, K, Hattori, M, Katayama, T, Hanada, S, Tamaki, H, Marumo, K, Maeda, H, Nedachi, M, Iwasaki, W, Suwa, Y & Sakata, S 2015, 'Tepidicaulis Marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions', International Journal of Systematic and Evolutionary Microbiology, 巻. 65, 番号 6, pp. 1749-1754. https://doi.org/10.1099/ijs.0.000167
Takeuchi, Mio ; Yamagishi, Takao ; Kamagata, Yoichi ; Oshima, Kenshiro ; Hattori, Masahira ; Katayama, Taiki ; Hanada, Satoshi ; Tamaki, Hideyuki ; Marumo, Katsumi ; Maeda, Hiroto ; Nedachi, Munetomo ; Iwasaki, Wataru ; Suwa, Yuichi ; Sakata, Susumu. / Tepidicaulis Marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions. :: International Journal of Systematic and Evolutionary Microbiology. 2015 ; 巻 65, 番号 6. pp. 1749-1754.
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abstract = "A moderately thermophilic, aerobic, stalked bacterium (strain MA2T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2Twas most closely related to the genera Rhodobium, Parvibaculum, and Rhodoligotrophos (92–93{\%} similarity) within the class Alphaproteobacteria. Strain MA2T was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16– 48 ºC (optimum growth at 42 ºC). This strain required yeast extract and NaCl (>1{\%}, w/v) for growth, tolerated up to 11{\%} (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C18: 1ω7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol{\%}. Strain MA2Tperformed denitrification and produced N2O from nitrate under strictly microaerobic conditions. Strain MA2Tpossessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2T(=NBRC 109643T=DSM 27167T) as the type strain of the species.",
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AU - Takeuchi, Mio

AU - Yamagishi, Takao

AU - Kamagata, Yoichi

AU - Oshima, Kenshiro

AU - Hattori, Masahira

AU - Katayama, Taiki

AU - Hanada, Satoshi

AU - Tamaki, Hideyuki

AU - Marumo, Katsumi

AU - Maeda, Hiroto

AU - Nedachi, Munetomo

AU - Iwasaki, Wataru

AU - Suwa, Yuichi

AU - Sakata, Susumu

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