Tetrameric jacalin as a receptor for field effect transistor biosensor to detect secretory IgA in human sweat

Hiroki Hayashi, Naoki Sakamoto, Sho Hideshima*, Yoshitaka Harada, Mika Tsuna, Shigeki Kuroiwa, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

*この研究の対応する著者

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Secretory immunoglobulin A (s-IgA), found in biological fluids, is useful for monitoring condition on mental health to prevent depression. In this study, the non-invasive detection of s-IgA in human sweat was demonstrated using field effect transistor (FET) biosensors modified with a plant lectin, jacalin, as a receptor. The s-IgA molecules were detected with greater sensitivity using the jacalin-immobilized FET biosensors as compared to the sensitivity shown by Fab-immobilized FET biosensors. Jacalin, which is a small lectin tetramer, has four glycan-binding sites and can capture a large number of s-IgA molecules within the charge-detectable region in terms of Debye length. Moreover, the jacalin-immobilized FET biosensor could detect s-IgA at concentrations ranging from 0.1 μg/mL to 100 μg/mL. Additionally, by using a filtration process to eliminate the interference of other components found in human sweat, our FET sensing system could specifically and quantitatively detect s-IgA. Therefore, our results show the utility of this device in monitoring mental stress.

本文言語English
論文番号114371
ジャーナルJournal of Electroanalytical Chemistry
873
DOI
出版ステータスPublished - 2020 9月 15

ASJC Scopus subject areas

  • 分析化学
  • 化学工学(全般)
  • 電気化学

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