Tetrameric jacalin as a receptor for field effect transistor biosensor to detect secretory IgA in human sweat

Hiroki Hayashi, Naoki Sakamoto, Sho Hideshima, Yoshitaka Harada, Mika Tsuna, Shigeki Kuroiwa, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

研究成果: Article

抜粋

Secretory immunoglobulin A (s-IgA), found in biological fluids, is useful for monitoring condition on mental health to prevent depression. In this study, the non-invasive detection of s-IgA in human sweat was demonstrated using field effect transistor (FET) biosensors modified with a plant lectin, jacalin, as a receptor. The s-IgA molecules were detected with greater sensitivity using the jacalin-immobilized FET biosensors as compared to the sensitivity shown by Fab-immobilized FET biosensors. Jacalin, which is a small lectin tetramer, has four glycan-binding sites and can capture a large number of s-IgA molecules within the charge-detectable region in terms of Debye length. Moreover, the jacalin-immobilized FET biosensor could detect s-IgA at concentrations ranging from 0.1 μg/mL to 100 μg/mL. Additionally, by using a filtration process to eliminate the interference of other components found in human sweat, our FET sensing system could specifically and quantitatively detect s-IgA. Therefore, our results show the utility of this device in monitoring mental stress.

元の言語English
記事番号114371
ジャーナルJournal of Electroanalytical Chemistry
873
DOI
出版物ステータスPublished - 2020 9 15

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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