The ability of bovine mitochondrial transfer RNAMet to decode AUG and AUA codons

C. Takemoto, T. Koike, T. Yokogawa, L. Benkowski, L. L. Spremulli, T. A. Ueda, K. Nishikawa, K. Watanabe*

*この研究の対応する著者

研究成果: Article査読

29 被引用数 (Scopus)

抄録

The ability of bovine mitochondrial tRNAMet with the anticodon f5CAU (where f5C is 5-formylcytidine) to decode AUG and AUA codons was examined in a codon-dependent ribosomal binding assay. The AUG codon stimulated the binding of Met-tRNAMet to mitochondrial ribosomes in the presence of EF-Tu/Tsmt. In contrast, the AUA codon did not promote the binding to mitochondrial Met-tRNA to the ribosome. To investigate the translation of the AUG and AUA codons more fully, an in vitro translation system from bovine liver mitochondria was developed. The activity of this system was greatly enhanced by the addition of 1 mM spermine and reached about half the activity observed with a comparable translational system from E. coli. Two types of mRNA containing either AUG or AUA codons were synthesized using T7 RNA polymerase to trans cribe their chemically synthesized genes. In the E coli system, the AUG-containing mRNA was translated as Met and the AUA-containing mRNA was translated as Ile. The AUG-containing mRNA but not the AUA-containing mRNA was translated as Met by the mitochondrial translational system. The process by which the AUA codon is translated as Met in the mitochondrial system remains to be clarified.

本文言語English
ページ(範囲)104-108
ページ数5
ジャーナルBiochimie
77
1-2
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 生化学

フィンガープリント

「The ability of bovine mitochondrial transfer RNA<sup>Met</sup> to decode AUG and AUA codons」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル