The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment

Naoki Aoyama, Asako Oka, Kumiko Kitayama, Hitoshi Kurumizaka, Masahiko Harata*

*この研究の対応する著者

    研究成果: Article査読

    26 被引用数 (Scopus)

    抄録

    The actin family consists of conventional actin and various actin-related proteins (Arps). Some of these Arps are localized in the nucleus, and a fraction of each of these nuclear Arps is functionally involved in chromatin remodeling and histone acetyltransferase complexes. On the other hand, in mitotic cells, the localization and function of the nuclear Arps are largely unknown. Human Arp8 (hArp8), an ortholog of yeast nuclear Arp8, was recently found to be associated with the hINO80-chromatin remodeling complex along with hArp5. Here we report that hArp8, but not hArp5, accumulates on mitotic chromosomes. This is the first example where a member of the actin family is found to be associated with mitotic chromosomes. Expression of truncated hArp8 proteins and depletion of endogenous hArp8 by RNA interference caused misalignment of mitotic chromosomes, suggesting that chromosome-associated hArp8 has a role in chromosome behavior. In contrast, depletion of hIno80 and hArp5 did not cause misalignment of chromosomes, suggesting that the role of hArp8 at mitotic chromosomes is independent of the activity of hINO80 complexes. These findings provide the first insight into a novel function of actin family members in mitosis.

    本文言語English
    ページ(範囲)859-868
    ページ数10
    ジャーナルExperimental Cell Research
    314
    4
    DOI
    出版ステータスPublished - 2008 2 15

    ASJC Scopus subject areas

    • 細胞生物学

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