TY - JOUR
T1 - The activity of isolated neurons and the modulatory state of an isolated nervous system represent a recent behavioural state
AU - Dyakonova, Varvara E.
AU - Hernadi, Laszlo
AU - Ito, Etsuro
AU - Dyakonova, Taisia L.
AU - Chistopolsky, Ilya A.
AU - Zakharov, Igor S.
AU - Sakharov, Dmitri A.
N1 - Publisher Copyright:
© 2015. Published by The Company of Biologists Ltd.
PY - 2015/4/15
Y1 - 2015/4/15
N2 - Behavioural/motivational state is known to influence nearly all aspects of physiology and behaviour. The cellular basis of behavioural state control is only partially understood.Our investigation, performed on the pond snail Lymnaea stagnalis whose nervous systemis useful for work on completely isolated neurons, provided several results related to this problem. First,we demonstrated that the behavioural state can produce long-term changes in individual neurons that persist even after neuron isolation from the nervous system. Specifically, we found that pedal serotonergic neurons that control locomotion show higher activity and lower membrane potential after being isolated from the nervous systems of hungry animals. Second, we showed that the modulatory state (the chemical neuroactive microenvironment of the central ganglia) changes in accordance with the nutritional state of an animal and produces predicted changes in single isolated locomotor neurons. Third,we report that observedhunger-inducedeffects canbeexplained by the increased synthesis of serotonin in pedal serotonergic neurons, which has an impact on the electrical activity of isolated serotonergic neurons and the intensity of extrasynaptic serotonin release from the pedal ganglia.
AB - Behavioural/motivational state is known to influence nearly all aspects of physiology and behaviour. The cellular basis of behavioural state control is only partially understood.Our investigation, performed on the pond snail Lymnaea stagnalis whose nervous systemis useful for work on completely isolated neurons, provided several results related to this problem. First,we demonstrated that the behavioural state can produce long-term changes in individual neurons that persist even after neuron isolation from the nervous system. Specifically, we found that pedal serotonergic neurons that control locomotion show higher activity and lower membrane potential after being isolated from the nervous systems of hungry animals. Second, we showed that the modulatory state (the chemical neuroactive microenvironment of the central ganglia) changes in accordance with the nutritional state of an animal and produces predicted changes in single isolated locomotor neurons. Third,we report that observedhunger-inducedeffects canbeexplained by the increased synthesis of serotonin in pedal serotonergic neurons, which has an impact on the electrical activity of isolated serotonergic neurons and the intensity of extrasynaptic serotonin release from the pedal ganglia.
KW - Extrasynaptic release
KW - Lymnaea stagnalis
KW - Neuromodulation
KW - Neurotransmitter synthesis
KW - Serotonergic neuron
KW - Volume transmission
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U2 - 10.1242/jeb.111930
DO - 10.1242/jeb.111930
M3 - Article
C2 - 25714568
AN - SCOPUS:84924201882
VL - 218
SP - 1151
EP - 1158
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
SN - 0022-0949
IS - 8
ER -