The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice

Yu Shin Sou, Satoshi Waguri, Jun Ichi Iwata, Takashi Ueno, Tsutomu Fujimura, Taichi Hara, Naoki Sawada, Akane Yamada, Noboru Mizushima, Yasuo Uchiyama, Eiki Kominami, Keiji Tanaka, Masaaki Komatsu*

*この研究の対応する著者

研究成果: Article査読

340 被引用数 (Scopus)

抄録

Autophagy is an evolutionarily conserved bulk-protein degradation pathway in which isolation membranes engulf the cytoplasmic constituents, and the resulting autophagosomes transport them to lysosomes. Two ubiquitin-like conjugation systems, termed Atg12 and Atg8 systems, are essential for autophagosomal formation. In addition to the pathophysio-logical roles of autophagy in mammals, recent mouse genetic studies have shown that the Atg8 system is predominantly under the control of the Atg12 system. To clarify the roles of the Atg8 system in mammalian autophagosome formation, we generated mice deficient in Atg3 gene encoding specific E2 enzyme for Atg8. Atg3-deficient mice were born but died within 1 d after birth. Conjugate formation of mammalian Atg8 homologues was completely defective in the mutant mice. Intriguingly, Atg12-Atg5 conjugation was markedly decreased in Atg3-deficient mice, and its dissociation from isolation membranes was significantly delayed. Furthermore, loss of Atg3 was associated with defective process of autophagosome formation, including the elongation and complete closure of the isolation membranes, resulting in malformation of the autophagosomes. The results indicate the essential role of the Atg8 system in the proper development of autophagic isolation membranes in mice.

本文言語English
ページ(範囲)4762-4775
ページ数14
ジャーナルMolecular biology of the cell
19
11
DOI
出版ステータスPublished - 2008 11 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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