The catalytic RNA of RNase P from Escherichia coli cleaves Drosophila 2S ribosomal RNA in vitro: A new type of naturally occurring substrate for the ribozyme

Yoshiaki Hori, Terumichi Tanaka, Yo Kikuchi*

*この研究の対応する著者

研究成果: Article査読

8 被引用数 (Scopus)

抄録

We have found that the catalytic RNA of RNase P of Escherichia coli (M1 RNA) can cleave 2S ribosomal RNA (2S rRNA) of Drosophila melanogaster at specific positions in vitro. The cleavage mainly occurred at two sites between nucleotides 11 and 12, and between 16 and 17 of 2S rRNA. Kinetic analyses of the reaction revealed that a dimer caused by intermolecular interaction of 2S rRNA may be the substrate for the cleavage between 11 and 12, while a simple monomer is the substrate for the cleavage between 16 and 17. Substrate recognition by M1 RNA is also discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)187-190
ページ数4
ジャーナルFEBS Letters
472
2-3
DOI
出版ステータスPublished - 2000 4 28
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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