The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA

Kozo Takeuchi, Tatsuya Nishino, Kouta Mayanagi, Naoki Horikoshi, Akihisa Osakabe, Hiroaki Tachiwana, Tetsuya Hori, Hitoshi Kurumizaka, Tatsuo Fukagawa

研究成果: Article

43 引用 (Scopus)

抜粋

The centromere is a specific genomic region upon which the kinetochore is formed to attach to spindle microtubules for faithful chromosome segregation. To distinguish this chromosomal region from other genomic loci, the centromere contains a specific chromatin structure including specialized nucleosomes containing the histone H3 variant CENP-A. In addition to CENP-A nucleosomes, we have found that centromeres contain a nucleosome-like structure comprised of the histone-fold CENP-T-W-S-X complex. However, it is unclear how the CENP-T-W-S-X complex associates with centromere chromatin. Here, we demonstrate that the CENP-T-W-S-X complex binds preferentially to ∼100 bp of linker DNA rather than nucleosome-bound DNA. In addition, we find that the CENP-T-W-S-X complex primarily binds to DNA as a (CENP-T-W-S-X)2 structure. Interestingly, in contrast to canonical nucleosomes that negatively supercoil DNA, the CENP-T-W-S-X complex induces positive DNA supercoils. We found that the DNA-binding regions in CENP-T or CENP-W, but not CENP-S or CENP-X, are required for this positive supercoiling activity and the kinetochore targeting of the CENP-T-W-S-X complex. In summary, our work reveals the structural features and properties of the CENP-T-W-S-X complex for its localization to centromeres.

元の言語English
ページ(範囲)1644-1655
ページ数12
ジャーナルNucleic Acids Research
42
発行部数3
DOI
出版物ステータスPublished - 2014 2

    フィンガープリント

ASJC Scopus subject areas

  • Genetics

これを引用

Takeuchi, K., Nishino, T., Mayanagi, K., Horikoshi, N., Osakabe, A., Tachiwana, H., Hori, T., Kurumizaka, H., & Fukagawa, T. (2014). The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA. Nucleic Acids Research, 42(3), 1644-1655. https://doi.org/10.1093/nar/gkt1124