The efficacy of basic fibroblast growth factor-loaded poly(lactic-co-glycolic acid) nanosheet for mouse wound healing

Shimpo Aoki, Mao Fujii, Toshinori Fujie, Keisuke Nishiwaki, Hiromi Miyazaki, Daizoh Saitoh, Shinji Takeoka, Tomoharu Kiyosawa, Manabu Kinoshita

研究成果: Article

8 引用 (Scopus)

抜粋

Although human recombinant basic fibroblast growth factor (bFGF) is widely used for wound healing, daily treatment with bFGF is required because of its short half-life. An effective controlled-release system of bFGF is, therefore, desired in clinical settings. To investigate the efficacy of a bFGF-loaded nanosheet for wound healing, focusing on the controlled-release of bFGF, bFGF-loaded poly(lactic-co-glycolic acid) (PGLA) nanosheets were developed, and their in vitro release profile of bFGF and their in vivo efficacy for wound healing were examined. A polyion complex of positively charged human recombinant bFGF and negatively charged alginate was sandwiched between PLGA nanosheets (70 nm thick for each layer). The resulting bFGF-loaded nanosheet robustly adhered to silicon skin by observation using a microscratch test. bFGF was gradually and continuously released over three days in an in vitro incubation study. Treatment with the bFGF-loaded nanosheets (every 3 day for 15 days) as well as with a conventional bFGF spray effectively promoted wound healing of mouse dorsal skin defects with accelerated tissue granulation and angiogenesis, although the dose of bFGF used in the treatment with the bFGF nanosheets was approximately 1/20 of the sprayed bFGF. In conclusion, we developed a bFGF-loaded nanosheet that sustained a continuous release of bFGF over three days and effectively promoted wound healing in mice.

元の言語English
ページ(範囲)1008-1016
ページ数9
ジャーナルWound Repair and Regeneration
25
発行部数6
DOI
出版物ステータスPublished - 2017 11 1

ASJC Scopus subject areas

  • Surgery
  • Dermatology

フィンガープリント The efficacy of basic fibroblast growth factor-loaded poly(lactic-co-glycolic acid) nanosheet for mouse wound healing' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Aoki, S., Fujii, M., Fujie, T., Nishiwaki, K., Miyazaki, H., Saitoh, D., Takeoka, S., Kiyosawa, T., & Kinoshita, M. (2017). The efficacy of basic fibroblast growth factor-loaded poly(lactic-co-glycolic acid) nanosheet for mouse wound healing. Wound Repair and Regeneration, 25(6), 1008-1016. https://doi.org/10.1111/wrr.12604