The ESC-E(Z) complex participates in the hedgehog signaling pathway

Norihisa Shindo, Atsushi Sakai, Daisuke Arai, Osamu Matsuoka, Yukihiko Yamasaki, Toru Higashinakagawa*

*この研究の対応する著者

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Polycomb group (PcG) genes are required for stable inheritance of epigenetic states throughout development, a phenomenon termed cellular memory. In Drosophila and mice, the product of the E(z) gene, one of the PcG genes, constitutes the ESC-E(Z) complex and specifically methylates histone H3. It has been argued that this methylation sets the stage for appropriate repression of certain genes. Here, we report the isolation of a well-conserved homolog of E(z), olezh2, in medaka. Hypomorphic knock-down of olezh2 resulted in a cyclopia phenotype and markedly perturbed hedgehog signaling, consistent with our previous report on oleed, a medaka esc. We also found cyclopia in embryos treated with trichostatin A, an inhibitor of histone deacetylase, which is a transient component of the ESC-E(Z) complex. The level of tri-methylation at lysine 27 of histone H3 was substantially decreased in both olezh2 and oleed knock-down embryos, and in embryos with hedgehog signaling perturbed by forskolin. We conclude that the ESC-E(Z) complex per se participates in hedgehog signaling.

本文言語English
ページ(範囲)1179-1187
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
327
4
DOI
出版ステータスPublished - 2005 2月 25
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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