The fabrication and testing of electrospun silica nanofiber membranes for the detection of proteins

P. H. Tsou*, C. K. Chou, S. M. Saldana, M. C. Hung, J. Kameoka

*この研究の対応する著者

研究成果: Article査読

26 被引用数 (Scopus)

抄録

In this study, we fabricated electrospun silica nanofiber membranes and investigated their use in biomolecular sensing. The diameter, porosity and surface-to-volume ratio of nanofiber membranes were investigated under different fabrication conditions. Using this type of nanofiber membrane, enzyme-linked immunosorbent assay (ELISA) was performed, and the results were compared with those obtained with conventional ELISA using polystyrene well plates. The minimum detectable concentration was determined as 0.19 ng ml-1 (1.6 pM), which is 32 times lower than that of conventional ELISA. In addition, the detection time for all processes for the nanofiber membrane was reduced to 1 h, compared with 1 day for conventional ELISA. The increased sensitivity, faster reaction time, and affordability of the nanofiber membrane make it well suited for bio-chip use.

本文言語English
論文番号445714
ジャーナルNanotechnology
19
44
DOI
出版ステータスPublished - 2008 11月 5
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 化学 (全般)
  • 材料科学(全般)
  • 材料力学
  • 機械工学
  • 電子工学および電気工学

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