TY - JOUR
T1 - The fabrication and testing of electrospun silica nanofiber membranes for the detection of proteins
AU - Tsou, P. H.
AU - Chou, C. K.
AU - Saldana, S. M.
AU - Hung, M. C.
AU - Kameoka, J.
PY - 2008/11/5
Y1 - 2008/11/5
N2 - In this study, we fabricated electrospun silica nanofiber membranes and investigated their use in biomolecular sensing. The diameter, porosity and surface-to-volume ratio of nanofiber membranes were investigated under different fabrication conditions. Using this type of nanofiber membrane, enzyme-linked immunosorbent assay (ELISA) was performed, and the results were compared with those obtained with conventional ELISA using polystyrene well plates. The minimum detectable concentration was determined as 0.19 ng ml-1 (1.6 pM), which is 32 times lower than that of conventional ELISA. In addition, the detection time for all processes for the nanofiber membrane was reduced to 1 h, compared with 1 day for conventional ELISA. The increased sensitivity, faster reaction time, and affordability of the nanofiber membrane make it well suited for bio-chip use.
AB - In this study, we fabricated electrospun silica nanofiber membranes and investigated their use in biomolecular sensing. The diameter, porosity and surface-to-volume ratio of nanofiber membranes were investigated under different fabrication conditions. Using this type of nanofiber membrane, enzyme-linked immunosorbent assay (ELISA) was performed, and the results were compared with those obtained with conventional ELISA using polystyrene well plates. The minimum detectable concentration was determined as 0.19 ng ml-1 (1.6 pM), which is 32 times lower than that of conventional ELISA. In addition, the detection time for all processes for the nanofiber membrane was reduced to 1 h, compared with 1 day for conventional ELISA. The increased sensitivity, faster reaction time, and affordability of the nanofiber membrane make it well suited for bio-chip use.
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U2 - 10.1088/0957-4484/19/44/445714
DO - 10.1088/0957-4484/19/44/445714
M3 - Article
AN - SCOPUS:58149240153
VL - 19
JO - Nanotechnology
JF - Nanotechnology
SN - 0957-4484
IS - 44
M1 - 445714
ER -