The genome landscape of the African Green Monkey kidney-derived vero cell line

Osada Naoki, Kohara Arihiro, Yamaji Toshiyuki, Hirayama Noriko, Kasai Fumio, Sekizuka Suyoshi, Kuroda Makoto, Hanada Kentaro*, Masahira Hattori

*この研究の対応する著者

研究成果: Article査読

103 被引用数 (Scopus)

抄録

Continuous cell lines that originate from mammalian tissues serve as not only invaluable tools for life sciences, but also important animal cell substrates for the production of various types of biological pharmaceuticals. Verocells aresusceptible to various types of microbes and toxins and have widely contributed to not only microbiology, but also the production of vaccines for human use. We here showed the genome landscape of a Vero cell line, in which 25,877 putative protein-coding genes were identified in the 2.97-Gb genome sequence. A homozygous ∼9-Mb deletion on chromosome 12 caused the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitor genes in Vero cells. In addition, an ∼59-Mb loss of heterozygosity around this deleted region suggested that the homozygosity of the deletion was established by a large-scale conversion. Moreover, a genomic analysis of Vero cells revealed a female Chlorocebus sabaeus origin and proviral variations of the endogenous simian type D retrovirus. These results revealed the genomic basis for the non-tumourigenic permanent Vero cell lineage susceptible to various pathogens and will be useful for generating new sub-lines and developing new tools in the quality control of Vero cells.

本文言語English
ページ(範囲)673-683
ページ数11
ジャーナルDNA Research
21
6
DOI
出版ステータスPublished - 2014 12 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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