抄録
Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.
本文言語 | English |
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ページ(範囲) | 756-761 |
ページ数 | 6 |
ジャーナル | Biochemical and Biophysical Research Communications |
巻 | 85 |
号 | 2 |
DOI | |
出版ステータス | Published - 1978 11月 29 |
外部発表 | はい |
ASJC Scopus subject areas
- 生化学
- 生物理学
- 分子生物学