The influence of temperature upon polysomes of spermatids of rat testes

Masahisa Nakamura, Peter F. Hall

研究成果: Article

10 引用 (Scopus)

抄録

Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.

元の言語English
ページ(範囲)756-761
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
85
発行部数2
DOI
出版物ステータスPublished - 1978 11 29
外部発表Yes

Fingerprint

Polyribosomes
Spermatids
Testis
Rats
Ribosomes
Temperature
Proteins
Cell-Free System
Phenylalanine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

The influence of temperature upon polysomes of spermatids of rat testes. / Nakamura, Masahisa; Hall, Peter F.

:: Biochemical and Biophysical Research Communications, 巻 85, 番号 2, 29.11.1978, p. 756-761.

研究成果: Article

Nakamura, Masahisa ; Hall, Peter F. / The influence of temperature upon polysomes of spermatids of rat testes. :: Biochemical and Biophysical Research Communications. 1978 ; 巻 85, 番号 2. pp. 756-761.
@article{c3326c9e0c09403e91b02098d9d01ad8,
title = "The influence of temperature upon polysomes of spermatids of rat testes",
abstract = "Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.",
author = "Masahisa Nakamura and Hall, {Peter F.}",
year = "1978",
month = "11",
day = "29",
doi = "10.1016/0006-291X(78)91225-1",
language = "English",
volume = "85",
pages = "756--761",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The influence of temperature upon polysomes of spermatids of rat testes

AU - Nakamura, Masahisa

AU - Hall, Peter F.

PY - 1978/11/29

Y1 - 1978/11/29

N2 - Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.

AB - Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.

UR - http://www.scopus.com/inward/record.url?scp=0018219128&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018219128&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(78)91225-1

DO - 10.1016/0006-291X(78)91225-1

M3 - Article

C2 - 736932

AN - SCOPUS:0018219128

VL - 85

SP - 756

EP - 761

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -