The influence of temperature upon polysomes of spermatids of rat testes

Masahisa Nakamura*, Peter F. Hall

*この研究の対応する著者

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.

本文言語English
ページ(範囲)756-761
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
85
2
DOI
出版ステータスPublished - 1978 11 29
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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