抄録
Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 756-761 |
| ページ数 | 6 |
| ジャーナル | Biochemical and Biophysical Research Communications |
| 巻 | 85 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 1978 11 29 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
これを引用
The influence of temperature upon polysomes of spermatids of rat testes. / Nakamura, Masahisa; Hall, Peter F.
：: Biochemical and Biophysical Research Communications, 巻 85, 番号 2, 29.11.1978, p. 756-761.研究成果: Article
}
TY - JOUR
T1 - The influence of temperature upon polysomes of spermatids of rat testes
AU - Nakamura, Masahisa
AU - Hall, Peter F.
PY - 1978/11/29
Y1 - 1978/11/29
N2 - Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.
AB - Incorporation of [3H]phenylalanine into protein by a reconstituted lysate subcellular system (ribosomes plus high-speed supernatant) from rat spermatids was measured at 34°C after 5 minutes preincubation of one component at 0°C while the other component was incubated at temperatures from 30°C to 40°C. Preincubation at temperatures above 34°C inhibits the ribosomal activity but not the high-speed supernatant activity. The incubation of lysate above 34°C results from a dissociation of polysomes to monosomes. These results indicate that ribosomes are the most sensitive component to the increased temperature on protein synthesis in lysate cell free system by spermatids and that the inhibition of protein synthesis in spermatids above 34°C is at least partly explained by the breakdown of polysomes in these cells.
UR - http://www.scopus.com/inward/record.url?scp=0018219128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018219128&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(78)91225-1
DO - 10.1016/0006-291X(78)91225-1
M3 - Article
C2 - 736932
AN - SCOPUS:0018219128
VL - 85
SP - 756
EP - 761
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -