The lifestyle of the segmented filamentous bacterium: A non-culturable gut-associated immunostimulating microbe inferred by whole-genome sequencing

Tomomi Kuwahara, Yositoshi Ogura, Kenshiro Oshima, Ken Kurokawa, Tadasuke Ooka, Hideki Hirakawa, Takehiko Itoh, Haruyuki Nakayama-Imaohji, Minoru Ichimura, Kikuji Itoh, Chieko Ishifune, Yoichi Maekawa, Koji Yasutomo, Masahira Hattori, Tetsuya Hayashi*

*この研究の対応する著者

研究成果: Article査読

66 被引用数 (Scopus)

抄録

Numerous microbes inhabit the mammalian intestinal track and strongly impact host physiology; however, our understanding of this ecosystem remains limited owing to the high complexity of the microbial community and the presence of numerous non-culturable microbes. Segmented filamentous bacteria (SFBs), which are clostridia-related Gram-positive bacteria, are among such non-culturable populations and are well known for their unique morphology and tight attachment to intestinal epithelial cells. Recent studies have revealed that SFBs play crucial roles in the post-natal maturation of gut immune function, especially the induction of Th17 lymphocytes. Here, we report the complete genome sequence of mouse SFBs. The genome, which comprises a single circular chromosome of 1 620 005 bp, lacks genes for the biosynthesis of almost all amino acids, vitamins/cofactors and nucleotides, but contains a full set of genes for sporulation/germination and, unexpectedly, for chemotaxis/flagella- based motility. These findings suggest a triphasic lifestyle of the SFB, which comprises two types of vegetative (swimming and epicellular parasitic) phases and a dormant (spore) phase. Furthermore, SFBs encode four types of flagellin, three of which are recognized by Toll-like receptor 5 and could elicit the innate immune response. Our results reveal the non-culturability, lifestyle and immunostimulation mechanisms of SFBs and provide a genetic basis for the future development of the SFB cultivation and gene-manipulation techniques.

本文言語English
ページ(範囲)291-303
ページ数13
ジャーナルDNA Research
18
4
DOI
出版ステータスPublished - 2011 8
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 分子生物学

フィンガープリント

「The lifestyle of the segmented filamentous bacterium: A non-culturable gut-associated immunostimulating microbe inferred by whole-genome sequencing」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル