The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo

Hiroyuki Kamiya*, Satoki Fukunaga, Takashi Ohyama, Hideyoshi Harashima

*この研究の対応する著者

研究成果: Article査読

19 被引用数 (Scopus)

抄録

The intranuclear disposition of a plasmid is extremely important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on plasmid expression were examined in vivo. A naked luciferase-plasmid was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. The location of the left-handedly curved sequence determined the transgene expression, without affecting the amount of intranuclear exogenous DNA. The plasmid containing the curved sequence at the location that results in the exposure of the TATA box out of the nucleosome core showed the highest expression. These results suggest that sequences with high histone affinity could control transgene expression from plasmids in vivo.

本文言語English
ページ(範囲)7-12
ページ数6
ジャーナルArchives of Biochemistry and Biophysics
461
1
DOI
出版ステータスPublished - 2007 5 1

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学

フィンガープリント

「The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル