The molecular characterization and in situ expression pattern of pea SCARECROW gene

Naomi Sassa, Yasuhiko Matsushita, Teruko Nakamura, Hiroshi Nyunoya*

*この研究の対応する著者

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library. Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues. A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct from the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR. A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript. Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.

本文言語English
ページ(範囲)385-394
ページ数10
ジャーナルPlant and Cell Physiology
42
4
DOI
出版ステータスPublished - 2001 4
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 植物科学
  • 細胞生物学

フィンガープリント

「The molecular characterization and in situ expression pattern of pea SCARECROW gene」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル