The nitric oxide/cyclic GMP pathway in the olfactory processing system of the terrestrial slug Limax marginatus

Sayoko Fujie, Hitoshi Aonuma, Iori Ito, Alan Gelperin, Etsuro Ito*

*この研究の対応する著者

研究成果: Article査読

33 被引用数 (Scopus)

抄録

To examine the distribution of nitric oxide (NO)-generative cells and NO-responsive cells in the tentacles and procerebral lobes (olfactory processing center) of terrestrial slugs, we applied NADPH diaphorase (NADPH-d) histochemistry and NO-induced cyclic GMP (cGMP)-like immunohistochemistry. We found that NADPH-d reactive cells/fibers and cGMP-like immunoreactive cells/fibers were different, but they were localized adjacent to each other, in both the tentacles and the procerebral lobes. Then, we measured the concentration of NO that was generated around the procerebral lobes using an NO sensitive electrode, when the olfactory nerve was electrically stimulated as a replacement for an odorant stimulus. Stimulation of the olfactory nerve evoked an increase in NO concentration at nanomolar levels, suggesting that binding of nanomolar concentrations of NO to the prosthetic heme group activates soluble guanylyl cyclase. Taken together with previously reported physiological data, our results, therefore, showed that the NO/cGMP pathways are involved in slug olfactory processing.

本文言語English
ページ(範囲)15-26
ページ数12
ジャーナルZoological Science
19
1
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 動物科学および動物学

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