The P3 domain of E. coli ribonuclease P RNA can be truncated and replaced

Terumichi Tanaka, Naomi Kanda, Yo Kikuchi

研究成果: Article

3 引用 (Scopus)

抜粋

We prepared some truncated and replaced P3 mutants of Escherichia coli RNase P RNA, and used them to examine the RNase P ribozyme and holoenzyme reactions of a pre-tRNA substrate. The results indicated that mutations in the P3 domain did not affect the cleavage site selection of the pre-tRNA substrate, but did affect the efficiency of cleavage of the substrate. Results of stepwise truncation of the P3 domain and its replacement by the TAR sequence showed that the P3 domain of the E. coli RNase P was able to be truncated to certain length and was replaceable, but could not be deleted in the ribozyme.

元の言語English
ページ(範囲)101-104
ページ数4
ジャーナルFEBS Letters
577
発行部数1-2
DOI
出版物ステータスPublished - 2004 11 5
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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