The primary structure of iodopsin, a chicken red-sensitive cone pigment

Osamu Kuwata, Yasushi Imamoto, Toshiyuki Okano, Koichi Kokame, Daisuke Kojima, Hiroki Matsumoto, Akihiro Morodome, Yoshitaka Fukada, Yoshinori Shichida, Kunio Yasuda, Yoshiro Shimura, Tôru Yoshizawa

研究成果: Article査読

65 被引用数 (Scopus)

抄録

A purified iodopsin was digested by CNBr or several proteolytic enzymes into fragments, the amino acid sequences of which were determined. A partial sequence of the C-terminal fragment was utilized for synthesizing an oligonucleotide probe which identified the iodopsin cDNA (1339 bases). The deduced amino acid sequence (362 residues) had 80%, 42% or 43% homology to that of human red-sensitive cone pigment, cattle or chicken rhodospin, respectively. Although the hydropathy profile implies that iodopsin, like rhodopsin, has 7 transmembrane α-helical segments, iodopsin may have a hydrophilic pocket near the seventh segment on the basis of the unexpected cleavages in the middle of the segment VII by chymotrypsin under nondenaturing conditions.

本文言語English
ページ(範囲)128-132
ページ数5
ジャーナルFEBS Letters
272
1-2
DOI
出版ステータスPublished - 1990 10 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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