The PURE system for protein production

Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, Takuya Ueda

研究成果: Chapter

23 被引用数 (Scopus)

抄録

In the field of molecular biology or biochemistry, preparation and use of purified proteins involved in a certain biological system is crucial for understanding their mechanisms and functions in cells or organisms. The recent progress in a cell-free translation system allows us to prepare proteins in a test tube directly from cDNAs that encode the amino acid sequences. The use of the reconstituted cell-free translation system termed PURE (Protein synthesis Using Recombinant Elements) for these purposes is effective in several applications. Here we describe methods of recombinant protein expression using the PURE system for molecular biological or biochemical studies.

本文言語English
ホスト出版物のタイトルCell-Free Protein Synthesis
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ275-284
ページ数10
ISBN(印刷版)9781627037815
DOI
出版ステータスPublished - 2014
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1118
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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