抄録
The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 74-77 |
| ページ数 | 4 |
| ジャーナル | FEBS Letters |
| 巻 | 514 |
| 号 | 1 |
| DOI | |
| 出版ステータス | Published - 2002 3月 6 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 生物理学
- 構造生物学
- 生化学
- 分子生物学
- 遺伝学
- 細胞生物学