The role of SmpB protein in trans-translation

Yoshihiro Shimizu, Takuya Ueda

研究成果: Article

54 引用 (Scopus)

抜粋

The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.

元の言語English
ページ(範囲)74-77
ページ数4
ジャーナルFEBS Letters
514
発行部数1
DOI
出版物ステータスPublished - 2002 3 6
外部発表Yes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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