抜粋
The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 74-77 |
| ページ数 | 4 |
| ジャーナル | FEBS Letters |
| 巻 | 514 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | Published - 2002 3 6 |
| 外部発表 | Yes |
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
フィンガープリント The role of SmpB protein in trans-translation' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。
これを引用
Shimizu, Y., & Ueda, T. (2002). The role of SmpB protein in trans-translation. FEBS Letters, 514(1), 74-77. https://doi.org/10.1016/S0014-5793(02)02333-5