The t-loop region of animal mitochondrial trna(agy) is a main recognition site for homologous seryl-tRNA synthetase

Takuya Ueda*, Yoshihisa Yotsumoto, Kenzi Ikeda, Kimitsuna Watanabe

*この研究の対応する著者

研究成果: Article査読

50 被引用数 (Scopus)

抄録

Recognition sites of bovine mitochondrial serine tRNA specific for codons AGY [tRNASer(AGY)] by the cognate mitochondrial seryl-tRNA synthetase were studied using a range of tRNASer(AGY) variants which were obtained by the in vitro transcription of synthetic tRNA genes with T7 RNA polymerase. Base replacements in the anticodon and discriminator sites did not affect serine acceptance. However, deletion and/or replacement in the T-loop region completely deprived the variants of their charging activities. Point mutation experiments in this region also showed that the adenosine residue in the middle of the T-loop (position 58), which is involved in tertiary interaction between the T-loop and the truncated D-arm [de Bruijn and Klug, 1983] played a significant role in the recognition process by the synthetase.

本文言語English
ページ(範囲)2217-2222
ページ数6
ジャーナルNucleic acids research
20
9
DOI
出版ステータスPublished - 1992 5月 11
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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