The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Elsa Kress, Françoise Schwager, René Holtackers, Jonas Seiler, François Prodon, Esther Zanin, Annika Eiteneues, Mika Toya, Asako Sugimoto, Hemmo Meyer, Patrick Meraladi, Monica Gotta

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Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.

元の言語English
ページ(範囲)559-575
ページ数17
ジャーナルJournal of Cell Biology
201
発行部数4
DOI
出版物ステータスPublished - 2013 5

ASJC Scopus subject areas

  • Cell Biology

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    Kress, E., Schwager, F., Holtackers, R., Seiler, J., Prodon, F., Zanin, E., Eiteneues, A., Toya, M., Sugimoto, A., Meyer, H., Meraladi, P., & Gotta, M. (2013). The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. Journal of Cell Biology, 201(4), 559-575. https://doi.org/10.1083/jcb.201209107