The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Elsa Kress, Françoise Schwager, René Holtackers, Jonas Seiler, François Prodon, Esther Zanin, Annika Eiteneues, Mika Toya, Asako Sugimoto, Hemmo Meyer, Patrick Meraladi, Monica Gotta*

*この研究の対応する著者

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.

本文言語English
ページ(範囲)559-575
ページ数17
ジャーナルJournal of Cell Biology
201
4
DOI
出版ステータスPublished - 2013 5月
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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