TY - JOUR
T1 - The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins
AU - Kikuchi, Yo
AU - Hirai, Keiko
AU - Hishinuma, Fumio
PY - 1984/7/25
Y1 - 1984/7/25
N2 - The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.
AB - The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.
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U2 - 10.1093/nar/12.14.5685
DO - 10.1093/nar/12.14.5685
M3 - Article
C2 - 6379603
AN - SCOPUS:0021770775
VL - 12
SP - 5685
EP - 5692
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 14
ER -