The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins

Yo Kikuchi*, Keiko Hirai, Fumio Hishinuma

*この研究の対応する著者

研究成果: Article査読

43 被引用数 (Scopus)

抄録

The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.

本文言語English
ページ(範囲)5685-5692
ページ数8
ジャーナルNucleic Acids Research
12
14
DOI
出版ステータスPublished - 1984 7 25
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 健康、毒物学および変異誘発
  • 毒物学
  • 遺伝学(臨床)
  • 遺伝学

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