Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy

T. Tojima, Y. Yamane, H. Takagi, T. Takeshita, T. Sugiyama, H. Haga, K. Kawabata, T. Ushiki, K. Abe, T. Yoshioka, Etsuro Ito

研究成果: Article

44 引用 (Scopus)

抄録

We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.

元の言語English
ページ(範囲)471-481
ページ数11
ジャーナルNeuroscience
101
発行部数2
DOI
出版物ステータスPublished - 2000 11 7
外部発表Yes

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Atomic Force Microscopy
Neurons
Syntaxin 1
Synaptophysin
Hybrid Cells
Synaptic Vesicles
Presynaptic Terminals
Neuroblastoma
Glioma
Electron Scanning Microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

これを引用

Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy. / Tojima, T.; Yamane, Y.; Takagi, H.; Takeshita, T.; Sugiyama, T.; Haga, H.; Kawabata, K.; Ushiki, T.; Abe, K.; Yoshioka, T.; Ito, Etsuro.

:: Neuroscience, 巻 101, 番号 2, 07.11.2000, p. 471-481.

研究成果: Article

Tojima, T, Yamane, Y, Takagi, H, Takeshita, T, Sugiyama, T, Haga, H, Kawabata, K, Ushiki, T, Abe, K, Yoshioka, T & Ito, E 2000, 'Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy', Neuroscience, 巻. 101, 番号 2, pp. 471-481. https://doi.org/10.1016/S0306-4522(00)00320-1
Tojima, T. ; Yamane, Y. ; Takagi, H. ; Takeshita, T. ; Sugiyama, T. ; Haga, H. ; Kawabata, K. ; Ushiki, T. ; Abe, K. ; Yoshioka, T. ; Ito, Etsuro. / Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy. :: Neuroscience. 2000 ; 巻 101, 番号 2. pp. 471-481.
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abstract = "We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.",
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AU - Yamane, Y.

AU - Takagi, H.

AU - Takeshita, T.

AU - Sugiyama, T.

AU - Haga, H.

AU - Kawabata, K.

AU - Ushiki, T.

AU - Abe, K.

AU - Yoshioka, T.

AU - Ito, Etsuro

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N2 - We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.

AB - We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.

KW - Exocytosis

KW - FM1-43

KW - NG108-15

KW - Scanning electron microscopy

KW - Synaptic vesicle

KW - Varicosity

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