抄録
We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 471-481 |
| ページ数 | 11 |
| ジャーナル | Neuroscience |
| 巻 | 101 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 2000 11 7 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
これを引用
Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy. / Tojima, T.; Yamane, Y.; Takagi, H.; Takeshita, T.; Sugiyama, T.; Haga, H.; Kawabata, K.; Ushiki, T.; Abe, K.; Yoshioka, T.; Ito, Etsuro.
：: Neuroscience, 巻 101, 番号 2, 07.11.2000, p. 471-481.研究成果: Article
}
TY - JOUR
T1 - Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy
AU - Tojima, T.
AU - Yamane, Y.
AU - Takagi, H.
AU - Takeshita, T.
AU - Sugiyama, T.
AU - Haga, H.
AU - Kawabata, K.
AU - Ushiki, T.
AU - Abe, K.
AU - Yoshioka, T.
AU - Ito, Etsuro
PY - 2000/11/7
Y1 - 2000/11/7
N2 - We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.
AB - We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100 nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells. (C) 2000 IBRO.
KW - Exocytosis
KW - FM1-43
KW - NG108-15
KW - Scanning electron microscopy
KW - Synaptic vesicle
KW - Varicosity
UR - http://www.scopus.com/inward/record.url?scp=0034619446&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034619446&partnerID=8YFLogxK
U2 - 10.1016/S0306-4522(00)00320-1
DO - 10.1016/S0306-4522(00)00320-1
M3 - Article
C2 - 11074169
AN - SCOPUS:0034619446
VL - 101
SP - 471
EP - 481
JO - Neuroscience
JF - Neuroscience
SN - 0306-4522
IS - 2
ER -