Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

Hideya Ohashi, Haruhiko Morita, Tomoyuki Tahara, Hikaru Tsunakawa, Atsushi Matsumoto, Kinya Ogami, Takashi Kato, Hiroshi Miyazaki

研究成果: Article

抜粋

We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.

元の言語English
ページ(範囲)199-206
ページ数8
ジャーナルCytotechnology
26
発行部数3
DOI
出版物ステータスPublished - 1998 1 1

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

これを引用

Ohashi, H., Morita, H., Tahara, T., Tsunakawa, H., Matsumoto, A., Ogami, K., Kato, T., & Miyazaki, H. (1998). Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22. Cytotechnology, 26(3), 199-206. https://doi.org/10.1023/A:1007915809529