Thyrotropin-Releasing Hormone (TRH) Is the Major Prolactin-Releasing Factor in the Bullfrog Hypothalamus

Keisuke Nakajima, Daisuke Uchida, Makoto Sakai, Noriyuki Takahashi, Tadashi Yanagisawa, Kazutoshi Yamamoto, Sakae Kikuyama

    研究成果: Article査読

    16 被引用数 (Scopus)

    抄録

    A substance exhibiting potent activity in stimulating the release of prolactin from bullfrog (Rana catesbeiana) pituitary in vitro was isolated from an acid extract of bullfrog hypothalami by gel-filtration chromatography (Sephadex G-15), ion-exchange chromatography (Mono-S HR 5/5), and reverse-phase high-performance liquid chromatography (TSK-gel ODS-120T). Its amino acid composition was similar to that of synthetic thyrotropin-releasing hormone (TRH). Radioimmunoassay confirmed that the substance had TRH immunoreactivity. Moreover, it exhibited the same chromatographic behavior as that of synthetic TRH. These results clearly indicate that the isolated hypothalamic substance is TRH, and that it is the major prolactin-releasing factor present in the bullfrog hypothalamus.

    本文言語English
    ページ(範囲)11-16
    ページ数6
    ジャーナルGeneral and Comparative Endocrinology
    89
    1
    DOI
    出版ステータスPublished - 1993 1

    ASJC Scopus subject areas

    • Endocrinology

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