Time-dependent absorption anisotropy and rotational diffusion of proteins in membranes.

S. Kawato, K. Kinosita

    研究成果: Article査読

    56 被引用数 (Scopus)

    抄録

    The decay of flash-induced absorption anisotropy, r(t), of a chromophore in a membrane protein is closely correlated with rotational diffusion of the protein in the membrane. We develop a theory of time-dependent absorption anisotropy which is applicable to both linear chromophores and planar chromophores which have two different absorption moments at right angles to one another. The theory treats two types of rotational diffusion of membrane proteins: one is rotation of the whole protein about the normal to the plane of the membrane, and the other is restricted wobbling of the whole or part of the protein molecule. In the former case, r(t) is determined by a rotational diffusion coefficient and an angle between the absorption moment(s) and the normal to the plane of the membrane. Rotation of rigid transmembrane proteins can be described by this treatment. In the latter case, r(t) is characterized by a wobbling diffusion coefficient and the degree of orientational constraint. This treatment may be applicable to independent wobbling of the hydrophilic part of membrane proteins. We further show that, for linear and circularly degenerate chromophores, the effect of the excitation flash intensity on r(t) can be accounted for by a constant scaling factor.

    本文言語English
    ページ(範囲)277-296
    ページ数20
    ジャーナルBiophysical Journal
    36
    1
    出版ステータスPublished - 1981 10

    ASJC Scopus subject areas

    • Biophysics

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