Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells

Koji Fukui, Keisuke Ushiki, Hirokatsu Takatsu, Tatsuro Koike, Shiro Urano

研究成果: Article

18 引用 (Scopus)

抄録

It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to induction of cell death. Here we found that treatment of cerebellar granule neurons (CGCs) with 0.5 μM hydrogen peroxide induced axonal injury, but not cell death. The number of dendrites remarkably decreased in hydrogen peroxide-treated CGCs, and extensive beading was observed on survival dendrites. In addition, an abnormal band of the original collapsin response mediator protein (CRMP)-2 was detected by Western blotting in hydrogen peroxide-treated CGCs. Treatment with each tocotrienol isoform prevented axonal and dendrite degeneration and induction of the abnormal band of the original band of CRMP-2 in hydrogen peroxide-treated CGCs. These results indicate that treatment with tocotrienols may therefore be neuroprotective in the presence of hydrogen peroxide by preventing changes to the CRMP-2 that occur before neuron death.

元の言語English
ページ(範囲)184-193
ページ数10
ジャーナルFree Radical Research
46
発行部数2
DOI
出版物ステータスPublished - 2012 2
外部発表Yes

Fingerprint

Tocotrienols
Dendrites
Hydrogen Peroxide
Axons
Cell death
Reactive Oxygen Species
Cell Death
Neurons
Disease Progression
Protein Isoforms
Western Blotting
Tissue
Wounds and Injuries
collapsin response mediator protein-2

ASJC Scopus subject areas

  • Biochemistry

これを引用

Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells. / Fukui, Koji; Ushiki, Keisuke; Takatsu, Hirokatsu; Koike, Tatsuro; Urano, Shiro.

:: Free Radical Research, 巻 46, 番号 2, 02.2012, p. 184-193.

研究成果: Article

Fukui, Koji ; Ushiki, Keisuke ; Takatsu, Hirokatsu ; Koike, Tatsuro ; Urano, Shiro. / Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells. :: Free Radical Research. 2012 ; 巻 46, 番号 2. pp. 184-193.
@article{d7526cd38c5f409e8466a2a562725db1,
title = "Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells",
abstract = "It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to induction of cell death. Here we found that treatment of cerebellar granule neurons (CGCs) with 0.5 μM hydrogen peroxide induced axonal injury, but not cell death. The number of dendrites remarkably decreased in hydrogen peroxide-treated CGCs, and extensive beading was observed on survival dendrites. In addition, an abnormal band of the original collapsin response mediator protein (CRMP)-2 was detected by Western blotting in hydrogen peroxide-treated CGCs. Treatment with each tocotrienol isoform prevented axonal and dendrite degeneration and induction of the abnormal band of the original band of CRMP-2 in hydrogen peroxide-treated CGCs. These results indicate that treatment with tocotrienols may therefore be neuroprotective in the presence of hydrogen peroxide by preventing changes to the CRMP-2 that occur before neuron death.",
keywords = "autophagy, axon, dendrite, ROS, tocotrienol",
author = "Koji Fukui and Keisuke Ushiki and Hirokatsu Takatsu and Tatsuro Koike and Shiro Urano",
year = "2012",
month = "2",
doi = "10.3109/10715762.2011.647689",
language = "English",
volume = "46",
pages = "184--193",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells

AU - Fukui, Koji

AU - Ushiki, Keisuke

AU - Takatsu, Hirokatsu

AU - Koike, Tatsuro

AU - Urano, Shiro

PY - 2012/2

Y1 - 2012/2

N2 - It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to induction of cell death. Here we found that treatment of cerebellar granule neurons (CGCs) with 0.5 μM hydrogen peroxide induced axonal injury, but not cell death. The number of dendrites remarkably decreased in hydrogen peroxide-treated CGCs, and extensive beading was observed on survival dendrites. In addition, an abnormal band of the original collapsin response mediator protein (CRMP)-2 was detected by Western blotting in hydrogen peroxide-treated CGCs. Treatment with each tocotrienol isoform prevented axonal and dendrite degeneration and induction of the abnormal band of the original band of CRMP-2 in hydrogen peroxide-treated CGCs. These results indicate that treatment with tocotrienols may therefore be neuroprotective in the presence of hydrogen peroxide by preventing changes to the CRMP-2 that occur before neuron death.

AB - It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to induction of cell death. Here we found that treatment of cerebellar granule neurons (CGCs) with 0.5 μM hydrogen peroxide induced axonal injury, but not cell death. The number of dendrites remarkably decreased in hydrogen peroxide-treated CGCs, and extensive beading was observed on survival dendrites. In addition, an abnormal band of the original collapsin response mediator protein (CRMP)-2 was detected by Western blotting in hydrogen peroxide-treated CGCs. Treatment with each tocotrienol isoform prevented axonal and dendrite degeneration and induction of the abnormal band of the original band of CRMP-2 in hydrogen peroxide-treated CGCs. These results indicate that treatment with tocotrienols may therefore be neuroprotective in the presence of hydrogen peroxide by preventing changes to the CRMP-2 that occur before neuron death.

KW - autophagy

KW - axon

KW - dendrite

KW - ROS

KW - tocotrienol

UR - http://www.scopus.com/inward/record.url?scp=84856256430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856256430&partnerID=8YFLogxK

U2 - 10.3109/10715762.2011.647689

DO - 10.3109/10715762.2011.647689

M3 - Article

C2 - 22149330

AN - SCOPUS:84856256430

VL - 46

SP - 184

EP - 193

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 2

ER -